Two dimensional gel electrophoresis of proteins. methods and applications

Two-dimensional gel electrophoresis and immunoblotting of Campylobacter pylori proteins.

Whole-cell, outer-membrane protein, flagellum-associated antigens and partially purified urease of Campylobacter pylori were analyzed by two-dimensional gel electrophoresis. C. pylori strains were readily distinguished from strains of Campylobacter jejuni, C. coli, and C. fetus by absence of major outer membrane proteins with Mrs of 41,000 to 45,000. C. pylori strains also lacked the acidic sur...

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis of cerebrospinal fluid proteins.

Two-dimensional electrophoresis, with isoelectric focusing in the first dimension and sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the second, has been adapted for the high-resolution analysis of cerebrospinal fluid proteins. Proteins were detected with a new, highly sensitive silver stain that made visible more than 300 polypeptides from 60 microL of spinal fluid, in highly rep...

Improved methods for separation of wheat endosperm proteins and analysis by two-dimensional gel electrophoresis

Fractionation of seed proteins has been a significant area of interest and activity, since Osborne (1924) introduced his solubility fractionation methods. In particular, the fractionation of wheat endosperm proteins has presented numerous challenges, because of their unique solubilities. Recently, we combined solubility fractionation and 2-DE protein analysis methods that taken separately may n...

Fluorescence two-dimensional difference gel electrophoresis for biomaterial applications.

Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between two or more conditions. In this review, we consider the application of DiGE-both minimal and saturation labelling-to biomaterials research, considering the challenges and rewards of this approach.